Disk Diffusion

This Medical Laboratory Science lesson in Medical Microbiology supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Perform disk diffusion AST with standardized inoculum, media, incubation, and zone measurement quality checks.

Advanced28 min read15 sectionsMedical Microbiology

Section 1 of 150%

Learning Objectives

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Section 1 of 15 · Getting started

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Getting started

Section 1 of 15

Learning Objectives

Each objective describes an AST bench competency, method, QC, and category assignment, not antimicrobial selection or dosing.

Sequence disk diffusion setup from inoculum preparation through medium inoculation, disk placement, incubation, and zone reading
Identify how inoculum density, Mueller–Hinton medium quality, disk spacing/potency, and incubation conditions alter zone diameters
Apply QC and institutional breakpoint tables as release gates before assigning interpretive categories