Overview
Type-specific HSV IgG assays detect antibodies to gG-1 and gG-2, distinguishing HSV-1 from HSV-2 exposure.
Clinical Importance
Assess HSV-2 exposure in selected counseling/serodiscordant pathways
Support timing questions when swabs for PCR are negative and clinical suspicion remains
Specimen Requirements
- Specimen: serum (preferred for most serology/autoimmune immunoassays) or plasma when the method is validated
- Tube: gold/SST (serum separator) or red-top serum; follow method-specific plasma acceptability
- Handling: allow SST to clot fully; centrifuge and separate promptly; avoid repeated freeze–thaw
- Storage: refrigerate short-term; freeze aliquots for delayed testing per manufacturer stability
- Stability: analyte- and kit-specific; do not exceed validated time/temperature limits
Analytical Method
ELISA/CLIA using gG antigens. Older non–type-specific assays are obsolete for type differentiation. HSV IgM is generally not recommended for genital herpes diagnosis.
Instruments Used
Automated analyzers
- Roche Cobas immunoassay systems
- Abbott Alinity / Architect immunoassay systems
- Siemens Healthineers Atellica / ADVIA Centaur systems
- DiaSorin LIAISON systems
- bioMérieux VIDAS systems
- Ortho Vitros immunoassay systems
Manual methods
- Manual ELISA and rapid immunochromatographic serology tests
Reference Values
Assay cutoffs, units, and reference intervals are method- and manufacturer-specific. Always interpret against the performing laboratory report (CLSI EP28-A3c for quantitative intervals where applicable). Serologic patterns require clinical correlation and current guideline algorithms (WHO/CDC/society guidance as adopted locally).
Index cutoffs are method-specific (negative/equivocal/positive). Equivocal zones require repeat testing per kit.
Interpretation
Common Causes of Increased Results
- HSV-2 IgG positive; prior HSV-2 infection
- HSV-1 IgG positive; prior HSV-1 infection (oral or genital)
Common Causes of Decreased Results
- Negative; no detectable type-specific IgG (window period possible)
Clinical Significance
- Selected serologic counseling scenarios
- TORCH-related panels when included
Factors Affecting Results
Pre-analytical
- Hemolysis, lipemia, or icterus (assay-dependent interference)
- Wrong tube type or incomplete clotting of SST
- Repeated freeze–thaw of serum aliquots
- Testing too early or too late relative to infection/vaccination timing for serology
Analytical
- Using non–type-specific HSV assays
- Ordering HSV IgM for genital lesions
Post-analytical
- Equating serology with the need for acute lesion PCR
Advantages and Limitations
Advantages
- Type-specific exposure documentation
Limitations
- Does not diagnose the current lesion; swab NAAT does
- HSV IgM not useful for most clinical decisions
Clinical Pearls
Swab the lesion for HSV PCR; type-specific IgG only writes the exposure history.
References
Authoritative textbooks, guidelines, and reviews supporting this laboratory test reference. Verify reference intervals, critical limits, and method details against institutional protocols and current guideline editions.
Textbooks
- McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods (verify current edition). Chapters on clinical immunology and serology.
- Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics (verify current edition). Immunoassay and protein chapters.
CLSI and Professional Guidelines
- Centers for Disease Control and Prevention. Laboratory testing recommendations for infectious disease serology and related algorithms (verify current CDC guidance for the pathogen).
- Clinical and Laboratory Standards Institute. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory. CLSI document EP28-A3c (verify current edition).
- Clinical and Laboratory Standards Institute. Immunoassay and related method evaluation documents (e.g., EP05, EP06, EP07, EP17; verify current editions relevant to the assay).
- College of American Pathologists. Accreditation and proficiency testing expectations for immunology/serology (verify current CAP checklist requirements).
- International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). Recommendations relevant to protein and immunoassay measurands (verify current IFCC documents).
WHO Publications
- World Health Organization. Laboratory and diagnostic guidance for serologic and immunologic testing (verify current WHO publication for the analyte/assay).
Frequently Asked Questions
Common questions about using this HSV-1 / HSV-2 Antibodies laboratory test Medical Laboratory Library reference in Medical Laboratory Science education.
What is the HSV-1 / HSV-2 Antibodies laboratory test reference used for?
This Medical Laboratory Library page summarizes the clinical importance of HSV-1 / HSV-2 Antibodies. Review the Clinical Importance section for why the test is ordered in laboratory medicine practice.
Where can I find HSV-1 / HSV-2 Antibodies specimen requirements?
See the Specimen Requirements section on this page for specimen information related to HSV-1 / HSV-2 Antibodies. Confirm collection details against your laboratory protocol.
Where are HSV-1 / HSV-2 Antibodies reference values listed?
See the Reference Values section on this Laboratory Library page. Reference ranges can vary by method and population, so verify intervals with your laboratory.
How do I interpret HSV-1 / HSV-2 Antibodies results?
Use the Interpretation section for laboratory interpretation framing, including increased and decreased result patterns when provided. Do not treat educational text as a patient-specific diagnosis.
What analytical method is used for HSV-1 / HSV-2 Antibodies?
See the Analytical Method section for principle and method notes. Method details may differ by instrument and institutional SOP.
Are related laboratory tests linked from HSV-1 / HSV-2 Antibodies?
Yes. The Related Laboratory Tests section lists connected references for continued lookup in the Medical Laboratory Library.
How can I continue learning after reading HSV-1 / HSV-2 Antibodies?
Use Continue learning links on this page for related Academy lessons, medical laboratory quizzes, or laboratory case studies when available for this Immunology & Serology topic.
What are the clinical pearls for HSV-1 / HSV-2 Antibodies?
See the Clinical Pearls section for concise laboratory practice notes associated with HSV-1 / HSV-2 Antibodies. Apply pearls alongside institutional protocols.
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