Search

PCR Reaction Components

This Medical Laboratory Science lesson in Molecular Diagnostics supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Identify the main polymerase chain reaction (PCR) reaction components used in clinical assays at a practical laboratory level.

Intermediate8 min read15 sectionsMolecular Diagnostics
Section 1 of 150%

Learning Objectives

Getting started

Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective is a clinical PCR skill for medical laboratory scientists, not advanced biochemistry.

  • Name the main polymerase chain reaction (PCR) reaction components used in clinical assays
  • State the practical role of primers, polymerase, nucleotides (deoxyribonucleoside triphosphates [dNTPs]), template, and buffer/magnesium (Mg)
  • Explain why missing or incorrect components can cause failed or invalid amplification

Section 1 of 15