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PCR Inhibition

This Medical Laboratory Science lesson in Molecular Diagnostics supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Recognize inhibited amplification during PCR, especially failed or weak internal controls, and never call a true negative from an inhibited or invalid reaction.

Intermediate8 min read15 sectionsMolecular Diagnostics
Section 1 of 150%

Learning Objectives

Getting started

Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective is a clinical PCR skill for medical laboratory scientists, not advanced biochemistry.

  • Recognize practical laboratory clues to PCR inhibition during amplification review
  • Relate failed or weak internal control (IC) performance to possible inhibition
  • Refuse to report a true negative when amplification validity is not supported

Section 1 of 15