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Digital PCR . Brief Clinical Overview

This Medical Laboratory Science lesson in Molecular Diagnostics supports laboratory science learning and laboratory interpretation for medical laboratory students and professionals.

Give brief clinical awareness of digital polymerase chain reaction (PCR): partitioning supports absolute quantification concepts, without advanced mathematics.

Intermediate7 min read15 sectionsMolecular Diagnostics
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Learning Objectives

Getting started

Section 1 of 15

Learning Objectives

Learning Objectives

Section: Learning Objectives

Each objective is a clinical PCR skill for medical laboratory scientists, not advanced biochemistry.

  • Describe digital polymerase chain reaction (dPCR) at a clinical awareness level as a method that partitions a sample into many small reactions
  • Explain the simple idea that counting positive partitions supports absolute quantification without relying on a standard curve in the same way as many real-time assays
  • State that whether dPCR is used, and how results are reported, is assay- and laboratory-specific

Section 1 of 15